Mutations of gyrA gene and parC gene in Streptococcus pneumoniae
نویسندگان
چکیده
منابع مشابه
Horizontal transfer of parC and gyrA in fluoroquinolone-resistant clinical isolates of Streptococcus pneumoniae.
We have analyzed genetically three clinical isolates (3180, 3870, and 1244) of Streptococcus pneumoniae with high-level ciprofloxacin resistance. Isolates 3180 and 3870 were atypical because of their insolubility in deoxycholate. However, they hybridized specifically with pneumococcal autolysin and pneumolysin gene probes and have typical pneumococcal atpC and atpA gene sequences. Analysis of t...
متن کاملAn extended PCR-RFLP assay for detection of parC, parE and gyrA mutations in fluoroquinolone-resistant Streptococcus pneumoniae.
Sir, In Gram-positive cocci, fluoroquinolone resistance is associated with mutational alterations in DNA gyrase and/or topoisomerase IV or with active efflux of the drugs. In Streptococcus pneumoniae, fluoroquinolone resistance is mainly due to substitutions in ParC (Ser-79→Phe or Tyr, Asp-83→Asn), ParE (Asp-435→Asn) or GyrA (Ser-81→Phe or Tyr, Glu-85→Lys or Gly).1,2 Therefore, detection of the...
متن کاملActivities of newer fluoroquinolones against Streptococcus pneumoniae clinical isolates including those with mutations in the gyrA, parC, and parE loci.
Resistance to fluoroquinolone (FQ) antibiotics in Streptococcus pneumoniae has been attributed primarily to specific mutations in the genes for DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). Resistance to some FQs can result from a single mutation in one or more of the genes encoding these essential enzymes. A group of 160 clinical isolates of pneumococci was examined in this ...
متن کاملAssociation between mutations in gyrA and parC genes of Acinetobacter baumannii clinical isolates and ciprofloxacin resistance
Objective(s): We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. Materials and Methods: Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyr...
متن کاملFirst Streptococcus agalactiae isolates highly resistant to quinolones, with point mutations in gyrA and parC.
Three isolates of Streptococcus agalactiae highly resistant to multiple fluoroquinolones were isolated in Japan. Compared with susceptible strains of S. agalactiae, these quinolone-resistant strains had double point mutations within the quinolone resistance-determining regions of gyrA and parC; Ser-81 was changed to Leu (TCA --> TTA) in the amino acid sequence deduced from gyrA, and Ser-79 was ...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 2003
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.77.133